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Addgene

pDD2603
(Plasmid #175087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1/Myc-His_A
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KSHV RTA phospho-mutant (ORF50)
  • Alt name
    195
  • Species
    Kaposi's sarcoma-associated herpesvirus 8
  • Mutation
    T504A, T508A
  • Entrez Gene
    ORF50 (a.k.a. HHV8GK18_gp52)

Cloning Information

  • Cloning method TOPO Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used genscript site-directed mutagenesis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDD2603 was a gift from Dirk Dittmer (Addgene plasmid # 175087 ; http://n2t.net/addgene:175087 ; RRID:Addgene_175087)

  • For your References section:

    The ORF45 Protein of Kaposi Sarcoma-Associated Herpesvirus Is an Inhibitor of p53 Signaling during Viral Reactivation. Alzhanova D, Meyo JO, Juarez A, Dittmer DP. J Virol. 2021 Nov 9;95(23):e0145921. doi: 10.1128/JVI.01459-21. Epub 2021 Sep 15. 10.1128/JVI.01459-21 PubMed 34523970
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