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Addgene

pDD2708
(Plasmid #175081)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KSHV ORF45
  • Alt name
    57
  • Species
    Kaposi's sarcoma-associated herpesvirus 8
  • Mutation
    RN1 mutant: V284A/L285A
  • Entrez Gene
    ORF45 (a.k.a. HHV8GK18_gp51)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Fanxiu Zhu (Florida State University), PMID: 19116250.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

KSHV ORF45 RN1 mutant (Li, 2009) was fused with C-terminal Flag and cloned into pcDNA3.1(+) using EcoRI and NotI sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDD2708 was a gift from Dirk Dittmer (Addgene plasmid # 175081 ; http://n2t.net/addgene:175081 ; RRID:Addgene_175081)

  • For your References section:

    The ORF45 Protein of Kaposi Sarcoma-Associated Herpesvirus Is an Inhibitor of p53 Signaling during Viral Reactivation. Alzhanova D, Meyo JO, Juarez A, Dittmer DP. J Virol. 2021 Nov 9;95(23):e0145921. doi: 10.1128/JVI.01459-21. Epub 2021 Sep 15. 10.1128/JVI.01459-21 PubMed 34523970
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