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Addgene

pCJ155
(Plasmid #175054)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175054 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Total vector size (bp) 5345
  • Vector type
    Unspecified
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRE
  • Insert Size (bp)
    1029
  • Promoter rbcLXS

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tggcgaagataagctctggt
  • 3′ sequencing primer gacacgacctccgaccac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCJ155 was a gift from David Nielsen (Addgene plasmid # 175054 ; http://n2t.net/addgene:175054 ; RRID:Addgene_175054)

  • For your References section:

    Exploiting Polyploidy for Markerless and Plasmid-Free Genome Engineering in Cyanobacteria. Jones CM, Parrish S, Nielsen DR. ACS Synth Biol. 2021 Sep 17;10(9):2371-2382. doi: 10.1021/acssynbio.1c00269. Epub 2021 Aug 17. 10.1021/acssynbio.1c00269 PubMed 34530614
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