p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D
(Plasmid
#175051)
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PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175051 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETDuet-1
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5420
- Total vector size (bp) 9849
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameReplication factor C subunit 5
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Alt nameUniprot P35250, P40938, P35249, P40937
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SpeciesH. sapiens (human)
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Insert Size (bp)4000
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Mutationchanged Thr76 to Asp, phosphomimetic mutation
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Entrez GeneRFC2 (a.k.a. RFC40)
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Entrez GeneRFC3 (a.k.a. RFC38)
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Entrez GeneRFC4 (a.k.a. A1, RFC37)
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Entrez GeneRFC5 (a.k.a. RFC36)
- Promoter T7 promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Rfc2-5specific (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D was a gift from Brian Kelch (Addgene plasmid # 175051 ; http://n2t.net/addgene:175051 ; RRID:Addgene_175051) -
For your References section:
Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch. Gaubitz C, Liu X, Magrino J, Stone NP, Landeck J, Hedglin M, Kelch BA. Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23571-23580. doi: 10.1073/pnas.2007437117. Epub 2020 Sep 9. 10.1073/pnas.2007437117 PubMed 32907938