p36-p37-p38-p40-pET-Duet-1
(Plasmid #175049)

• Purpose: Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli
• Depositing Lab: Brian Kelch
• Publication: Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23571-23580. doi: 10.1073/pnas.2007437117. Epub 2020 Sep 9.

Backbone

• Vector backbone: pETDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5420
• Total vector size (bp): 9849
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Replication factor C subunits 2-5
• Alt name: Uniprot P35250, P40938, P35249, P40937
• Species: H. sapiens (human)
• Insert Size (bp): 4000
• Entrez Gene: RFC4 (a.k.a. A1, RFC37)
• Entrez Gene: RFC2 (a.k.a. RFC40)
• Entrez Gene: RFC3 (a.k.a. RFC38)
• Entrez Gene: RFC5 (a.k.a. RFC36)
• Promoter: T7 promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Rfc2-5specific

Resource Information

• Supplemental Documents:
  • pET-DUET-hRfc2-5.gb
• A portion of this plasmid was derived from a plasmid made by: Cloned by Kadyrov et al. 10.1073/pnas.0903654106 Plasmids graciously provided by Drs M. Hedglin and Dr. S. J. Benkovic

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry