MS2_SC2_S_Beta
(Plasmid
#175046)
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PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Beta SARS-CoV-2 S gene (Amino Acid 319-869)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175046 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepMX
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 2200
- Total vector size (bp) 6264
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameMaturation Protein
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Alt nameA-Protein
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SpeciesMS2 Phage
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Insert Size (bp)1182
- Promoter T7
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer GATGTGCTGCAAGGCGATTAAGTTG
- 3′ sequencing primer ctatggaaaaacgccagcaacg (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameCoat Protein Dimer
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SpeciesSynthetic
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Insert Size (bp)801
- Promoter T7
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer GATGTGCTGCAAGGCGATTAAGTTG
- 3′ sequencing primer ctatggaaaaacgccagcaacg (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameS gene
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SpeciesSARS-CoV-2
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Insert Size (bp)1654
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MutationBeta (Amino Acid 319-869)
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GenBank IDYP_009724390.1
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
- Promoter T7
Cloning Information for Gene/Insert 3
- Cloning method Restriction Enzyme
- 5′ cloning site Esp3I (BsmBI) (destroyed during cloning)
- 3′ cloning site Esp3I (BsmBI) (destroyed during cloning)
- 5′ sequencing primer TGTCGAACAGAAAGTAATCGTATTGTAC
- 3′ sequencing primer CAGCTGGCACGACAGGTTTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MS2_SC2_S_Beta was a gift from Paul Freemont (Addgene plasmid # 175046 ; http://n2t.net/addgene:175046 ; RRID:Addgene_175046)