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pCDF-1b-RFC140ΔN555
(Plasmid #175043)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175043 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF-1b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3559
  • Total vector size (bp) 5344
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Replication factor C subunit 1
  • Alt name
    P140
  • Alt name
    UniProtKB - P35251
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1785
  • Entrez Gene
    RFC1 (a.k.a. A1, CANVAS, MHCBFB, PO-GA, RECC1, RFC, RFC140)
  • Promoter T7 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Originally cloned by Kadyrov et al. 0.1073/pnas.0903654106 Graciously provided to our lab by Drs M. Hedglin and S. J. Benkovic (Pennsylvania State University)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF-1b-RFC140ΔN555 was a gift from Brian Kelch (Addgene plasmid # 175043 ; http://n2t.net/addgene:175043 ; RRID:Addgene_175043)

  • For your References section:

    Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch. Gaubitz C, Liu X, Magrino J, Stone NP, Landeck J, Hedglin M, Kelch BA. Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23571-23580. doi: 10.1073/pnas.2007437117. Epub 2020 Sep 9. 10.1073/pnas.2007437117 PubMed 32907938
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