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Addgene

pPB-MPP8-112–858_Flag2-Blast
(Plasmid #175039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Mammalian Expression ; piggyBac
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mphosph8
  • Alt name
    AU040635, 1500035L22Rik, 4930548G07Rik
  • Species
    M. musculus (mouse)
  • Mutation
    Expression of MPP8 protein from amino acid position E112 to Q858
  • Entrez Gene
    Mphosph8 (a.k.a. 1500035L22Rik, 4930548G07Rik, AU040635)
  • Promoter CAG
  • Tag / Fusion Protein
    • Flag2 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-MPP8-112–858_Flag2-Blast was a gift from Kristian Helin (Addgene plasmid # 175039 ; http://n2t.net/addgene:175039 ; RRID:Addgene_175039)

  • For your References section:

    MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells. Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K. Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4. 10.1038/s41467-021-23308-4 PubMed 34031396
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