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pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST
(Plasmid #175018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCD5
  • Backbone manufacturer
    -
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MC1061
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    OK-HEF s57a ED
  • Species
    Synthetic
  • Mutation
    s57a esterase knock out mutant
  • GenBank ID
    MG720235
  • Promoter CMV
  • Tag / Fusion Protein
    • GCN4-TEV-sfGFP-TwinStrep (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG C
  • 3′ sequencing primer TTC AGG GGG AGG TGT GGG AG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST was a gift from Robert de Vries (Addgene plasmid # 175018 ; http://n2t.net/addgene:175018 ; RRID:Addgene_175018)

  • For your References section:

    Tissue Microarrays to Visualize Influenza D Attachment to Host Receptors in the Respiratory Tract of Farm Animals. Nemanichvili N, Berends AJ, Wubbolts RW, Grone A, Rijks JM, de Vries RP, Verheije MH. Viruses. 2021 Mar 31;13(4). pii: v13040586. doi: 10.3390/v13040586. 10.3390/v13040586 PubMed 33807137
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