pcDNA3-Akt-Scramble
(Plasmid #175014)

• Purpose: Negative-control construct for Akt-STOPS.
• Depositing Lab: Jin Zhang
• Publication: Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi: 10.1038/s41467-020-19937-w.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5517
• Total vector size (bp): 6405
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-Akt-Scramble
• Insert Size (bp): 888
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-Akt-Scramble.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry