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pcDNA3-Akt-STOPS-NLS
(Plasmid #175007)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175007 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 6399
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-Akt-STOPS-NLS
  • Insert Size (bp)
    927
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)
    • mCherry (N terminal on insert)
    • Nuclear localization signal (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Akt-STOPS-NLS was a gift from Jin Zhang (Addgene plasmid # 175007 ; http://n2t.net/addgene:175007 ; RRID:Addgene_175007)

  • For your References section:

    Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus. Zhou X, Zhong Y, Molinar-Inglis O, Kunkel MT, Chen M, Sun T, Zhang J, Shyy JY, Trejo J, Newton AC, Zhang J. Nat Commun. 2020 Nov 30;11(1):6088. doi: 10.1038/s41467-020-19937-w. 10.1038/s41467-020-19937-w PubMed 33257668
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