pBabe puro c-met WT
(Plasmid #17493)

• Depositing Lab: Joan Brugge
• Publication: Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

Backbone

• Vector backbone: pBabe puro
• Backbone manufacturer: Available at Addgene (#1764)
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: c-Met
• Species: M. musculus (mouse)
• Insert Size (bp): 4200
• Entrez Gene: Met (a.k.a. HGF, HGFR, Par4, c-Met)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pBABE-5'
• 3′ sequencing primer: pBABE-3'

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry