pLenti CMV TetR Blast (716-1)
(Plasmid #17492)

• Purpose: 3rd gen lentiviral Tetracycline repressor expression vector, CMV promoter, Blast 
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 8477
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tetracycline repressor
• Alt name: TetR
• Insert Size (bp): 647

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMVforw

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TetR gene derived from pLenti6/TR plasmid (Invitrogen)
• Articles Citing this Plasmid:
  • 42 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry