pAS_MmaPylT_EF1_NES-MmaPylRS AF
(Plasmid #174902)

• Purpose: expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
• Depositing Lab: Simon Elsaesser
• Publication: Elsaesser plasmids 2021 (unpublished)

Backbone

• Vector backbone: pAS
• Backbone manufacturer: Addgene #140008 based on PB510B-1 (System Bioscience)
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mma PylRS
• Species: Methanosarcina mazei
• Insert Size (bp): 1392
• Mutation: Y306 Y384 F
• Entrez Gene: pylS (a.k.a. MSMAC_RS17105, MSMAC_3241)
• Promoter: EF1
• Tag / Fusion Protein:
  • FLAG and NES (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pas_4xh7skpylt_ef1_nes-pylrs-af.gb
• A portion of this plasmid was derived from a plasmid made by: NES-Mma PylRS first described by Nikic et al. 2016, PMID: 27804198

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry