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Addgene

pAS_MmaPylT_EF1_NES-MmaPylRS WT
(Plasmid #174901)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174901 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS
  • Backbone manufacturer
    Addgene #140008 based on PB510B-1 (System Bioscience)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mma PylRS
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    1392
  • Entrez Gene
    pylS (a.k.a. MSMAC_RS17105, MSMAC_3241)
  • Promoter EF1
  • Tag / Fusion Protein
    • FLAG and NES (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    NES-Mma PylRS first described by Nikic et al. 2016, PMID: 27804198

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_MmaPylT_EF1_NES-MmaPylRS WT was a gift from Simon Elsaesser (Addgene plasmid # 174901 ; http://n2t.net/addgene:174901 ; RRID:Addgene_174901)
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