pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP
(Plasmid #174893)

• Purpose: amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette
• Depositing Lab: Simon Elsaesser
• Publication: Elsaesser plasmids 2021 (unpublished)

Backbone

• Vector backbone: pAS
• Backbone manufacturer: Addgene #140008 based on PB510B-1 (System Bioscience)
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry and EGFP
• Species: Aequorea victoria
• Insert Size (bp): 1488
• Promoter: EF1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pas_4xmmapylt_ef1_mcherry_tag_egfp.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry