pQCXIN X2/pTER shLUC (w347-1)
(Plasmid #17489)

• Purpose: Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Backbone

• Vector backbone: pQCXIN
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 7821
• Vector type: Retroviral, RNAi
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10F', 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Firefly Luciferase shRNA
• Alt name: shLUC
• Insert Size (bp): 66

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (destroyed during cloning)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

shRNA oligo sequence - 5'-CCTTACGCTGAGTACTTCGAGTGTGCTGTCCTCGAAGTACTCAGCGTAAGTTT