pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR
(Plasmid
#174883)
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PurposeTargeting vector for generating Pou5f1 STREAMING-tag KI cell
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174883 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEB2
- Total vector size (bp) 10491
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Vector typeMouse Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSTREAMING-tag with homology arms for Pou5f1
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SpeciesSynthetic
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GenBank ID
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13-fwr
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR was a gift from Hiroshi Ochiai (Addgene plasmid # 174883 ; http://n2t.net/addgene:174883 ; RRID:Addgene_174883) -
For your References section:
STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity. Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H. Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2. 10.1038/s41467-022-35286-2 PubMed 36539402
