pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP
(Plasmid
#174876)
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PurposePiggyBac transposon vector for expression of RNAPII Ser2ph mintbody
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174876 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPB-LR5
- Total vector size (bp) 8835
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Vector typeMammalian Expression ; piggyBac vector
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRNAPII Ser2ph mintbody-SNAP
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SpeciesSynthetic
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GenBank ID
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP was a gift from Hiroshi Ochiai (Addgene plasmid # 174876 ; http://n2t.net/addgene:174876 ; RRID:Addgene_174876) -
For your References section:
STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity. Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H. Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2. 10.1038/s41467-022-35286-2 PubMed 36539402