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Addgene

pCMV-Hs.cargo(Cas9)
(Plasmid #174864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 10649
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 flanked by HsPeg10 UTRs
  • Species
    H. sapiens (human); S.pyogenes
  • Insert Size (bp)
    5200
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA-SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Hs.cargo(Cas9) was a gift from Feng Zhang (Addgene plasmid # 174864 ; http://n2t.net/addgene:174864 ; RRID:Addgene_174864)

  • For your References section:

    Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery. Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae RK, Koonin EV, Zhang F. Science. 2021 Aug 20;373(6557):882-889. doi: 10.1126/science.abg6155. 10.1126/science.abg6155 PubMed 34413232
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