pCMV-MmPeg10rc4
(Plasmid #174858)

• Purpose: Expresses recoded mouse Peg10 in mammalian cells
• Depositing Lab: Feng Zhang
• Publication: Segel et al Science. 2021 Aug 20;373(6557):882-889. doi: 10.1126/science.abg6155.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 7074
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: recoded Mus musculus PEG10
• Species: M. musculus (mouse)
• Insert Size (bp): 2897
• GenBank ID: NM_130877.2
• Entrez Gene: Peg10 (a.k.a. AA407948, Edr, HB-1, MEF3L, Mar2, Mart2, MyEF-3, Rtl2)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • pCMV-MmPeg10_rc4.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene verified sequence is slightly different from the sequence reported in the publication. The plasmid works as described and an update map is included in the supplemental documents section above.