KM-CashGem-Xyl2-URA3-LINEAR
(Plasmid #174838)

• Purpose: pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus
• Depositing Lab: Zengyi Shao
• Publication: Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038/s41589-021-00893-5. doi: 10.1038/s41589-021-00893-5.

Backbone

• Vector backbone: pRS414
• Total vector size (bp): 13404
• Vector type: Bacterial Expression, Yeast Expression, CRISPR, Synthetic Biology
• Selectable markers: TRP1, URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Codon optimized Cas9:hGem
• Species: Synthetic; K. marxianus
• Insert Size (bp): 4503
• Promoter: TEF1p
• Tag / Fusion Protein:
  • hGeminin tag

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: atggacaagaagtactctatcg

Resource Information

• Supplemental Documents:
  • KM-CashGem-Xyl2-URA3-LINEAR
• A portion of this plasmid was derived from a plasmid made by: A portion of this plasmid was obtained from pIW447-CRISPR XYL2 (Addgene #98906)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry