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Addgene

pCS105
(Plasmid #174830)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174830 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1-TOPO
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 3956
  • Total vector size (bp) 5903
  • Vector type
    Bacterial Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    11R Unique
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1949
  • Mutation
    Changing TEL11R to a unique end
  • Promoter lac promoter
  • Tag / Fusion Protein
    • GAL4 5XUAS

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS105 was a gift from Carol Greider (Addgene plasmid # 174830 ; http://n2t.net/addgene:174830 ; RRID:Addgene_174830)

  • For your References section:

    Chromosome-specific telomere lengths and the minimal functional telomere revealed by nanopore sequencing. Sholes SL, Karimian K, Gershman A, Kelly TJ, Timp W, Greider CW. Genome Res. 2022 Apr;32(4):616-628. doi: 10.1101/gr.275868.121. Epub 2021 Oct 26. 10.1101/gr.275868.121 PubMed 34702734
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