Skip to main content
Addgene

pET41sIII_GST-ALC1-E175Q
(Plasmid #174814)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174814 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET41
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ALC1-E175Q
  • Species
    H. sapiens (human)
  • Mutation
    E175Q
  • Entrez Gene
    CHD1L (a.k.a. ALC1, CHDL)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protein residues 1-897 (a start codon included in-frame).

Please note: Plasmid contains R25P and S743C natural variants in ALC1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET41sIII_GST-ALC1-E175Q was a gift from Joan Conaway (Addgene plasmid # 174814 ; http://n2t.net/addgene:174814 ; RRID:Addgene_174814)

  • For your References section:

    Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling. Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn MP, Unruh JR, Florens L, Conaway RC, Conaway JW. Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118. 10.1073/pnas.2107277118 PubMed 34465625
Related items: