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Addgene

pCS2+WBP2NL-HA
(Plasmid #174791)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174791 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4100
  • Total vector size (bp) 5048
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    WBP2 N-terminal like
  • Alt name
    WBP2NL
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    942
  • Mutation
    aa#165, Y to H; aa# 287, D to H
  • GenBank ID
    BC022546.1
  • Entrez Gene
    wbp2nl.L (a.k.a. XELAEV_18023638mg, pawp, wbp2nl)
  • Promoter SP6
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+WBP2NL-HA was a gift from Sally Moody (Addgene plasmid # 174791 ; http://n2t.net/addgene:174791 ; RRID:Addgene_174791)

  • For your References section:

    Wbp2nl has a developmental role in establishing neural and non-neural ectodermal fates. Marchak A, Grant PA, Neilson KM, Datta Majumdar H, Yaklichkin S, Johnson D, Moody SA. Dev Biol. 2017 Sep 1;429(1):213-224. doi: 10.1016/j.ydbio.2017.06.025. Epub 2017 Jun 27. 10.1016/j.ydbio.2017.06.025 PubMed 28663133
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