p35u4
(Plasmid #174786)

• Purpose: Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system
• Depositing Lab: Katherine Berry
• Publication: Wang et al RNA. 2021 Apr;27(4):513-526. doi: 10.1261/rna.077404.120. Epub 2021 Jan 26.

Backbone

• Vector backbone: pACYC184
• Total vector size (bp): 3423
• Modifications to backbone: a 113bp deletion is present upstream of constitutive promoter; this plasmid was selected in a mutagenesis screen.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MS2 coat protein
• Alt name: MS2cp
• Species: Bacteriophage MS2
• Insert Size (bp): 378
• Mutation: V30I, A81G, positions 68-80 deleted (VATQTVGGVELPV), cloned behind a EFPGIHPGM linker
• Promoter: Synthetic Constitutive Promoter
• Tag / Fusion Protein:
  • λCI

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CAGACCAAAACGATCTCAAGAAGATCATC

Resource Information

• Supplemental Documents:
  • 174786_p35u4_pAC_CI-MS2cp_constitutive.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry