pZA71
(Plasmid
#174785)
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PurposeIn planta gene expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174785 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepICH86988
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Backbone manufacturerhttps://www.addgene.org/48076/
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker
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SpeciesN. benthamiana
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MutationWT
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZA71 was a gift from Sophien Kamoun (Addgene plasmid # 174785 ; http://n2t.net/addgene:174785 ; RRID:Addgene_174785) -
For your References section:
A vector system for fast-forward studies of the HOPZ-ACTIVATED RESISTANCE1 (ZAR1) resistosome in the model plant Nicotiana benthamiana. Harant A, Pai H, Sakai T, Kamoun S, Adachi H. Plant Physiol. 2022 Jan 20;188(1):70-80. doi: 10.1093/plphys/kiab471. 10.1093/plphys/kiab471 PubMed 34633454
