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Addgene

pZA54
(Plasmid #174767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICSL01005
  • Backbone manufacturer
    https://www.addgene.org/47996/
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GG_NbZAR1syn-D481V without STOP codon
  • Species
    N. benthamiana
  • Mutation
    D481V

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZA54 was a gift from Sophien Kamoun (Addgene plasmid # 174767 ; http://n2t.net/addgene:174767 ; RRID:Addgene_174767)

  • For your References section:

    A vector system for fast-forward studies of the HOPZ-ACTIVATED RESISTANCE1 (ZAR1) resistosome in the model plant Nicotiana benthamiana. Harant A, Pai H, Sakai T, Kamoun S, Adachi H. Plant Physiol. 2022 Jan 20;188(1):70-80. doi: 10.1093/plphys/kiab471. 10.1093/plphys/kiab471 PubMed 34633454
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