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PurposeRetroviral Luciferase expression vector, CMV/TO promoter, Puro
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQCXP
- Backbone size w/o insert (bp) 7189
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Vector typeMammalian Expression, Retroviral, Luciferase
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10F', 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFirefly Luciferase
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Insert Size (bp)1652
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVforw (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Luciferase gene derived from the pGL3 plasmid (Stratagene)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQCXP CMV/TO LUC (w432-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17476 ; http://n2t.net/addgene:17476 ; RRID:Addgene_17476) -
For your References section:
A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394
Map uploaded by the depositor.
