pPRIChp-hOtub1
(Plasmid
#174743)
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PurposeGene overexpression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 174743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPRIChp
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOTUB1
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SpeciesH. sapiens (human)
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Entrez GeneOTUB1 (a.k.a. HSPC263, OTB1, OTU1)
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPRIChp-hOtub1 was a gift from Shao-Cong Sun (Addgene plasmid # 174743 ; http://n2t.net/addgene:174743 ; RRID:Addgene_174743) -
For your References section:
The deubiquitinase Otub1 controls the activation of CD8(+) T cells and NK cells by regulating IL-15-mediated priming. Zhou X, Yu J, Cheng X, Zhao B, Manyam GC, Zhang L, Schluns K, Li P, Wang J, Sun SC. Nat Immunol. 2019 Jul;20(7):879-889. doi: 10.1038/s41590-019-0405-2. Epub 2019 Jun 10. 10.1038/s41590-019-0405-2 PubMed 31182807