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Addgene

pPRIChp-hOtub1
(Plasmid #174743)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174743 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pPRIChp
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OTUB1
  • Species
    H. sapiens (human)
  • Entrez Gene
    OTUB1 (a.k.a. HSPC263, OTB1, OTU1)
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPRIChp-hOtub1 was a gift from Shao-Cong Sun (Addgene plasmid # 174743 ; http://n2t.net/addgene:174743 ; RRID:Addgene_174743)
  • For your References section:

    The deubiquitinase Otub1 controls the activation of CD8(+) T cells and NK cells by regulating IL-15-mediated priming. Zhou X, Yu J, Cheng X, Zhao B, Manyam GC, Zhang L, Schluns K, Li P, Wang J, Sun SC. Nat Immunol. 2019 Jul;20(7):879-889. doi: 10.1038/s41590-019-0405-2. Epub 2019 Jun 10. 10.1038/s41590-019-0405-2 PubMed 31182807