pFendoNA2S
(Plasmid #174730)

• Purpose: Expresses GFP-endoNA2-S706A fusion protein for detection of polysialic acid. The endoNA2-S706A is a noncatalytic endosialidase that lacks the ability for releasing its C-terminal chaperone domain.
• Depositing Lab: Jukka Finne
• Publication: Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-7163.MCT-20-1031. doi: 10.1158/1535-7163.MCT-20-1031.

Backbone

• Vector backbone: pFEndoNA2
• Backbone manufacturer: Finne lab
• Backbone size w/o insert (bp): 7102
• Total vector size (bp): 7102
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: endoNA2-S706A
• Species: Escherichia phage PK1A2
• Insert Size (bp): 2403
• Mutation: Changed Serine 706 to Alanine
• GenBank ID: MG004687.1

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CCCGAAAAGTGCCACCTG
• 3′ sequencing primer: GTTCTGAGGTCATTACTGG

Resource Information

• Supplemental Documents:
  • pFendoNA2S.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be amplified and maintained in ampicillin-sensitive E. coli strains that harbor the lacIq mutation (such as XL1 Blue and JM109) or carries the pREP4 repressor plasmid (such as M15 [pREP4]).