pDsEndoNA2
(Plasmid #174728)

• Purpose: Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.
• Depositing Lab: Jukka Finne
• Publication: Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 10.1038/s41467-017-02057-3.

Backbone

• Vector backbone: pFEndoNA2
• Backbone manufacturer: Finne lab
• Backbone size w/o insert (bp): 7102
• Total vector size (bp): 7051
• Modifications to backbone: The egfp gene deleted, and NotI and SpeI restriction sites introduced
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DsRed-Monomer
• Alt name: DsRed. M1
• Species: Discosoma sp.
• Insert Size (bp): 686
• GenBank ID: EU827527.1
• Promoter: T5
• Tag / Fusion Protein:
  • Histag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: CCCGAAAAGTGCCACCTG

Resource Information

• Supplemental Documents:
  • pDsEndoNA2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be amplified and maintained in ampicillin-sensitive E. coli strains that harbor the lacIq mutation (such as XL1 Blue and JM109) or carries the pREP4 repressor plasmid (such as M15 [pREP4]).