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Purpose(Empty Backbone) Bifidobacterium-E.coli shuttle vector for seamless gene cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKO403
- Backbone size (bp) 4506
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Modifications to backboneThe lacZ gene was inserted into the plasmid backbone using infusion. Two Type IIS restriction sites were added in the 3' and 5' ends of the lacZ gene. The spectinomycin resistance gene was clarified from SapI restriction sites by silent mutagenesis
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsaddition of 2% x-gal to culture media is important for white blue selection
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Copy numberHigh Copy
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKO403_lacZ_Sp was a gift from Tohru Suzuki (Addgene plasmid # 174726 ; http://n2t.net/addgene:174726 ; RRID:Addgene_174726) -
For your References section:
Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium. Altaib H, Ozaki Y, Kozakai T, Sakaguchi K, Nomura I, Suzuki T. Microbiol Resour Announc. 2022 Jan 20;11(1):e0088421. doi: 10.1128/mra.00884-21. Epub 2022 Jan 13. 10.1128/mra.00884-21 PubMed 35023783
Map uploaded by the depositor.
