pKO403_lacZ_Cm
(Plasmid #174725)

• Purpose: (Empty Backbone) Bifidobacterium-E.coli shuttle vector for seamless gene cloning
• Depositing Lab: Tohru Suzuki
• Publication: Altaib et al Microbiol Resour Announc. 2022 Jan 20;11(1):e0088421. doi: 10.1128/mra.00884-21. Epub 2022 Jan 13.

Backbone

• Vector backbone: pKO403
• Backbone size (bp): 4305
• Modifications to backbone: The lacZ gene was inserted into the plasmid backbone using infusion. Two Type IIS restriction sites were added in the 3' and 5' ends of the lacZ gene. The chloramphenicol resistance gene was inserted in the plasmid backbone using infusion
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Growth instructions: addition of 2% x-gal to culture media is important for white blue selection
• Copy number: High Copy

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid runs as a dimer.