pFEndoNA
(Plasmid #174724)

• Purpose: Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.
• Depositing Lab: Jukka Finne
• Publication: Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149-60. doi: 10.1016/j.jim.2004.10.006. Epub 2004 Nov 14.

Backbone

• Vector backbone: pQE-31
• Backbone manufacturer: QIAGEN
• Backbone size w/o insert (bp): 3463
• Total vector size (bp): 7102
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP-endoNA fusion protein
• Species: Aequorea victoria, Yersinia enterocolitica, Escherichia phage PK1A
• Insert Size (bp): 3637
• GenBank ID: U76561.1, X13882.1, EF507428.1
• Promoter: T5
• Tag / Fusion Protein:
  • Histag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: CCCGAAAAGTGCCACCTG

Resource Information

• Supplemental Documents:
  • pFEndoNA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be amplified and maintained in ampicillin-sensitive E. coli strains that harbor the lacIq mutation (such as XL1 Blue and JM109) or carries the pREP4 repressor plasmid (such as M15 [pREP4]).