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pDRM98 mKate2 Blast
(Plasmid #174720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRLSIN.cPPT.MND
  • Backbone size w/o insert (bp) 6685
  • Total vector size (bp) 7087
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mKate2
  • Species
    Synthetic
  • Insert Size (bp)
    693
  • Promoter MND
  • Tags / Fusion Proteins
    • SV40 NLS with GGS linker (N terminal on backbone)
    • P2A linker (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    BSD
  • Alt name
    Blasticidin-S deaminase/BlastR
  • Species
    Aspergillus terreus
  • Insert Size (bp)
    393
  • GenBank ID
  • Promoter MND (off mKate-P2A)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDRM98 mKate2 Blast was a gift from Wayne Tilley (Addgene plasmid # 174720 ; http://n2t.net/addgene:174720 ; RRID:Addgene_174720)

  • For your References section:

    Selective inhibition of CDK9 in triple negative breast cancer. Mustafa EH, Laven-Law G, Kikhtyak Z, Nguyen V, Ali S, Pace AA, Iggo R, Kebede A, Noll B, Wang S, Winter JM, Dwyer AR, Tilley WD, Hickey TE. Oncogene. 2023 Nov 24. doi: 10.1038/s41388-023-02892-3. 10.1038/s41388-023-02892-3 PubMed 38001268
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