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H2B-mScarlet- hRec8(297-506)-hMeikin(1-332, E151R, R154E)- mNeonGreen
(Plasmid #174716)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174716 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-Blast
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rec8
  • Species
    H. sapiens (human)
  • Mutation
    hRec8: 297-506; hMeikin: 1-332, E151R, R154E
  • Entrez Gene
    REC8 (a.k.a. HR21spB, REC8L1, Rec8p)
  • Tags / Fusion Proteins
    • H2B-mScarlet (N terminal on insert)
    • mNeonGreen (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pNM869, Rec8 is fused to human Meikin fragment containing Separase-resistant mutations (aa1-332, E151R, R154E) . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H2B-mScarlet- hRec8(297-506)-hMeikin(1-332, E151R, R154E)- mNeonGreen was a gift from Iain Cheeseman (Addgene plasmid # 174716 ; http://n2t.net/addgene:174716 ; RRID:Addgene_174716)

  • For your References section:

    Separase cleaves the kinetochore protein Meikin at the meiosis I/II transition. Maier NK, Ma J, Lampson MA, Cheeseman IM. Dev Cell. 2021 Aug 9;56(15):2192-2206.e8. doi: 10.1016/j.devcel.2021.06.019. Epub 2021 Jul 30. 10.1016/j.devcel.2021.06.019 PubMed 34331869
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