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3xEGFP-mMeikin(1-391) - hRad21(142-275, E169R, R172E) - mMeikin(387-434)
(Plasmid #174713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Meikin
  • Species
    M. musculus (mouse)
  • Mutation
    mMeikin: 1-391; hRad21: 142-275, E169R, R172E; mMeikin: 387-434
  • Entrez Gene
    Meikin (a.k.a. 4930404A10Rik)
  • Tag / Fusion Protein
    • 3xEGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pNM963, compatible with in vitro transcription from SP6 promoter, C-terminal Separase cleavage site is derived from human Rad21 (aa142-275) with Separase resistant mutations (E169R, R172E), kinetochore localization is maintainted by C-terminal mMeikin fragment (aa387-434) . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xEGFP-mMeikin(1-391) - hRad21(142-275, E169R, R172E) - mMeikin(387-434) was a gift from Iain Cheeseman (Addgene plasmid # 174713 ; http://n2t.net/addgene:174713 ; RRID:Addgene_174713)

  • For your References section:

    Separase cleaves the kinetochore protein Meikin at the meiosis I/II transition. Maier NK, Ma J, Lampson MA, Cheeseman IM. Dev Cell. 2021 Aug 9;56(15):2192-2206.e8. doi: 10.1016/j.devcel.2021.06.019. Epub 2021 Jul 30. 10.1016/j.devcel.2021.06.019 PubMed 34331869
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