3xEGFP-mMeikin(1-391, E156R, R159E) - hRad21(142-275) - mMeikin(387-434)
(Plasmid
#174712)
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PurposeMammalian expression of mouse Meikin with wild-type Separase cleavage site eliminated and C-terminal Separase cleavage site inserted
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMeikin
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SpeciesM. musculus (mouse)
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MutationmMeikin: 1-391, E156R, R159E; hRad21: 142-275; mMeikin: 387-434
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Entrez GeneMeikin (a.k.a. 4930404A10Rik)
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Tag
/ Fusion Protein
- 3xEGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pNM962, compatible with in vitro transcription from SP6 promoter, C-terminal Separase cleavage site is derived from human Rad21 (aa142-275), kinetochore localization is maintainted by C-terminal mMeikin fragment (aa387-434) . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3xEGFP-mMeikin(1-391, E156R, R159E) - hRad21(142-275) - mMeikin(387-434) was a gift from Iain Cheeseman (Addgene plasmid # 174712 ; http://n2t.net/addgene:174712 ; RRID:Addgene_174712) -
For your References section:
Separase cleaves the kinetochore protein Meikin at the meiosis I/II transition. Maier NK, Ma J, Lampson MA, Cheeseman IM. Dev Cell. 2021 Aug 9;56(15):2192-2206.e8. doi: 10.1016/j.devcel.2021.06.019. Epub 2021 Jul 30. 10.1016/j.devcel.2021.06.019 PubMed 34331869