Lenti-evoA1-seBEC
(Plasmid
#174702)
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PurposeSmall-molecule controlled base editing
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174702 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLenti-evoA1-seBEC
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SpeciesH. sapiens (human), R. norvegicus (rat)
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MutationUGI-E11K
- Promoter EFS
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Tag
/ Fusion Protein
- flag tag (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-evoA1-seBEC was a gift from Rahul Kohli (Addgene plasmid # 174702 ; http://n2t.net/addgene:174702 ; RRID:Addgene_174702) -
For your References section:
Controllable genome editing with split-engineered base editors. Berrios KN, Evitt NH, DeWeerd RA, Ren D, Luo M, Barka A, Wang T, Bartman CR, Lan Y, Green AM, Shi J, Kohli RM. Nat Chem Biol. 2021 Dec;17(12):1262-1270. doi: 10.1038/s41589-021-00880-w. Epub 2021 Oct 18. 10.1038/s41589-021-00880-w PubMed 34663942