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Addgene

CMV-A3A-seBE4max-IRES
(Plasmid #174698)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174698 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A3A-seBE4max-IRES
  • Species
    H. sapiens (human)
  • Mutation
    A3A-M13I, UGI-E11K
  • Entrez Gene
    APOBEC3A (a.k.a. A3A, ARP3, PHRBN, bK150C2.1)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: NotI (unknown if destroyed), 3' Cloning Site: XmaI (unknown if destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-A3A-seBE4max-IRES was a gift from Rahul Kohli (Addgene plasmid # 174698 ; http://n2t.net/addgene:174698 ; RRID:Addgene_174698)

  • For your References section:

    Controllable genome editing with split-engineered base editors. Berrios KN, Evitt NH, DeWeerd RA, Ren D, Luo M, Barka A, Wang T, Bartman CR, Lan Y, Green AM, Shi J, Kohli RM. Nat Chem Biol. 2021 Dec;17(12):1262-1270. doi: 10.1038/s41589-021-00880-w. Epub 2021 Oct 18. 10.1038/s41589-021-00880-w PubMed 34663942
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