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Mucin MUC1-5TR-pET15b
(Plasmid #174694)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174694 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15B
  • Backbone manufacturer
    Novagen EMD Milipore
  • Backbone size w/o insert (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends BL21(DE3) cells for bacterial expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MUC1-TR
  • Alt name
    five copies of the human MUC1 tandem repeat (MUC1-5TR)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    329
  • Mutation
    none
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • hexahistidine (HIS) tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mucin MUC1-5TR-pET15b was a gift from Jean Gariepy (Addgene plasmid # 174694 ; http://n2t.net/addgene:174694 ; RRID:Addgene_174694)

  • For your References section:

    Nuclear magnetic resonance-based dissection of a glycosyltransferase specificity for the mucin MUC1 tandem repeat. Brokx RD, Revers L, Zhang Q, Yang S, Mal TK, Ikura M, Gariepy J. Biochemistry. 2003 Dec 2;42(47):13817-25. doi: 10.1021/bi0353070. 10.1021/bi0353070 PubMed 14636048
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