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Affibody EGFR:1907.BirA*-pET301/NT-DEST
(Plasmid #174691)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET301/NT-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7382
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends BL21(DE3) cells for bacterial expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
  • Alt name
    Anti EGFR affibody.BirA*
  • Species
    Staphylococcus aureus and Escherichia coli
  • Insert Size (bp)
    1237
  • Mutation
    R118G mutation in BirA
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • HIS tag (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Affibody EGFR:1907.BirA*-pET301/NT-DEST was a gift from Jean Gariepy (Addgene plasmid # 174691 ; http://n2t.net/addgene:174691 ; RRID:Addgene_174691)

  • For your References section:

    Labeling Cell Surface Receptors with Ligand.BirA* Bispecifics. Alwash M, Gariepy J. ACS Pharmacol Transl Sci. 2022 Feb 1;5(2):62-69. doi: 10.1021/acsptsci.1c00192. eCollection 2022 Feb 11. 10.1021/acsptsci.1c00192 PubMed 36742360
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