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6xHis-MBP-huErCas12a
(Plasmid #174685)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174685 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Backbone manufacturer
    Scott Gradia Lab
  • Backbone size w/o insert (bp) 6462
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use of strain Rosetta™ 2(DE3)pLysS for expression is highly recommended
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    huErCas12a
  • Alt name
    huErCpf1
  • Species
    Eubacterium rectale
  • Insert Size (bp)
    3840
  • Mutation
    Q926R mutation is observed as indicated from the lab the gene was obtained, but the mutation does not affect ErCas12a function
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis, MBP, TEV, NLS (N terminal on insert)
    • NLS, 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The vector is obtained from Scott Gradia Lab (Addgene #29656) while the gene is obtained from Stephen Ekker Lab (Addgene #132641) . Read the preprint on medRxiv here: https://www.medrxiv.org/content/10.1101/2021.07.21.21260653v1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6xHis-MBP-huErCas12a was a gift from Piyush Jain (Addgene plasmid # 174685 ; http://n2t.net/addgene:174685 ; RRID:Addgene_174685)

  • For your References section:

    A Combinatorial Approach towards Adaptability of 22 Functional Cas12a Orthologs for Nucleic Acid Detection in Clinical Samples. Nguyen LT, Macaluso NC, Jain PK. medRxiv 2021.07.21 10.1101/2021.07.21.21260653
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