pCH1 - pBait-empty
(Plasmid
#174663)
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Purpose(Empty Backbone) arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174663 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDF and pBAD
- Backbone size (bp) 3901
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCH1 - pBait-empty was a gift from Katherine Berry (Addgene plasmid # 174663 ; http://n2t.net/addgene:174663 ; RRID:Addgene_174663) -
For your References section:
Genetic identification of the functional surface for RNA binding by Escherichia coli ProQ. Pandey S, Gravel CM, Stockert OM, Wang CD, Hegner CL, LeBlanc H, Berry KE. Nucleic Acids Res. 2020 May 7;48(8):4507-4520. doi: 10.1093/nar/gkaa144. 10.1093/nar/gkaa144 PubMed 32170306
