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pGL4.10/RSV_SF3B3-miniXon_Luciferase
(Plasmid #174660)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174660 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.10[luc2]
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4242
  • Total vector size (bp) 5261
  • Modifications to backbone
    Removed BamHI site in backbone plasmid
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miniXon cassette
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    595
  • Mutation
    Kozak ATG initiation sequence in middle exon
  • Promoter RSV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamhI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer TCCCGTCTTCGAGTGGGTA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.10/RSV_SF3B3-miniXon_Luciferase was a gift from Beverly L. Davidson (Addgene plasmid # 174660 ; http://n2t.net/addgene:174660 ; RRID:Addgene_174660)

  • For your References section:

    Regulated control of gene therapies by drug-induced splicing. Monteys AM, Hundley AA, Ranum PT, Tecedor L, Muehlmatt A, Lim E, Lukashev D, Sivasankaran R, Davidson BL. Nature. 2021 Jul 28. pii: 10.1038/s41586-021-03770-2. doi: 10.1038/s41586-021-03770-2. 10.1038/s41586-021-03770-2 PubMed 34321659
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