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Addgene

pET28-FnCas12a-TEV
(Plasmid #174658)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174658 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FnCas12a
  • Species
    Francisella novicida

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-FnCas12a-TEV was a gift from Jin Wang & Huimin Zhao (Addgene plasmid # 174658 ; http://n2t.net/addgene:174658 ; RRID:Addgene_174658)

  • For your References section:

    Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination. Enghiad B, Huang C, Guo F, Jiang G, Wang B, Tabatabaei SK, Martin TA, Zhao H. Nat Commun. 2021 Feb 19;12(1):1171. doi: 10.1038/s41467-021-21275-4. 10.1038/s41467-021-21275-4 PubMed 33608525
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