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Purpose(Empty Backbone) CAPTURE method receiver plasmid for Streptomyces heterologous expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174655 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneThe plasmid is built using multiple fragments
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBE45 was a gift from Huimin Zhao (Addgene plasmid # 174655 ; http://n2t.net/addgene:174655 ; RRID:Addgene_174655) -
For your References section:
Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination. Enghiad B, Huang C, Guo F, Jiang G, Wang B, Tabatabaei SK, Martin TA, Zhao H. Nat Commun. 2021 Feb 19;12(1):1171. doi: 10.1038/s41467-021-21275-4. 10.1038/s41467-021-21275-4 PubMed 33608525
