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pLVX-IRES-hygro-EGFP-DFCP1
(Plasmid #174651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-DFCP1
  • Alt name
    ZFYVE1, PPP1R172, SR3, TAFF1, ZNFN2A1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3087
  • Entrez Gene
    ZFYVE1 (a.k.a. DFCP1, PPP1R172, SR3, TAFF1, ZNFN2A1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal plasmid ID: AJ212 bioRxiv 2021.04.21

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-IRES-hygro-EGFP-DFCP1 was a gift from Lukas Kapitein (Addgene plasmid # 174651 ; http://n2t.net/addgene:174651 ; RRID:Addgene_174651)

  • For your References section:

    Direct observation of aggregate-triggered selective autophagy in human cells. Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC. J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 10.1242/jcs.258824. Epub 2021 Oct 6. 10.1242/jcs.258824 PubMed 34447998
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