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Addgene

pBA-mKeima-PIM
(Plasmid #174650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    B-actin
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
  • Species
    Synthetic
  • Insert Size (bp)
    2687
  • Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second homo-FKBP domain
  • Promoter Chicken beta-actin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal plasmid ID: GK21 bioRxiv 2021.04.21

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA-mKeima-PIM was a gift from Lukas Kapitein (Addgene plasmid # 174650 ; http://n2t.net/addgene:174650 ; RRID:Addgene_174650)

  • For your References section:

    Direct observation of aggregate-triggered selective autophagy in human cells. Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC. J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 10.1242/jcs.258824. Epub 2021 Oct 6. 10.1242/jcs.258824 PubMed 34447998
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